However, this time it has been announced that the O2 will not be fined for the offence because Greenwich Council was "satisfied" the incident was a one-off.
The maximum fine for such an offence is £2,500 and it came into force on 1 July.
A spokeswoman for the O2 venue is reported to have said: "A band member appeared to have a cigarette, however it was extinguished almost faster than the message to put it out got to stage.
And the spokeswoman added: "Along with the rest of the country, we take the smoking ban seriously."
Greenwich Council said: "We have received no complaints from members of the public. We have reminded the O2 of their obligation to enforce the smoking legislation."
It was the first of the Rolling Stones- three gigs at the venue, which used to be known as the Millennium Dome.
